Fig. 6

ROS-dependent activation of the MAPK/ERK pathway by 1,5-AG in pre-B ALL. Western blotting (A) and statistical analyses (B) of p-ERK1/2 and ERK1/2 expressions of pre-B ALL cell lines with different doses of 1,5-AG. C–F p-ERK1/2 and ERK1/2 expressions were reversed by NAC and vitamin C treatment in pre-B ALL cells. The final concentration of 1,5-AG was 0.8 mM. Glyceraldehyde 3-phosphate dehydrogenase was used as a loading control. Original blots are presented in Supplementary Information 2. G–H The images present p-ERK1/2 staining in the spleen and BM from pre-B ALL mice with the indicated treatments (n = 5/group). Scale bars: 100 μm. Data are represented as means ± standard deviations. ns (P ≥ 0.05), ^*P < 0.05, ^**P < 0.01, ^***P < 0.001