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Fig. 4 | BMC Cancer

Fig. 4

From: 1,5-Anhydroglucitol promotes pre-B acute lymphocytic leukemia progression by driving glycolysis and reactive oxygen species formation

Fig. 4

1,5-AG promotes pre-B ALL cells proliferation by inducing cellular reactive oxygen species (ROS) production. A The proportion of CD19+ cells from peripheral blood-mononuclear cells (PB-MNCs) in three patients with pre-B ALL was detected by flow cytometry. B PB-MNCs were treated with different doses of 1,5-AG for 24 h to determine the cell viability. C ROS levels detected after 1,5-AG intervention for 24 h. D–E Cells were treated with 0.8 mM 1,5-AG with 2 mM NAC, and the ROS-positive cells were then measured and analyzed by flow cytometry. F–H Effects of 0.8 mM 1,5-AG with 2 mM NAC on cell viability (F), cell cycle (G), and apoptosis (H) in Reh and HAL-01 cells. I-J Effects of 0.8 mM 1,5-AG with 0.2 mM Vitamin C on cell viability (I) and ROS levels (J). Data are represented as means ± standard deviations. ns (P ≥ 0.05), *P < 0.05, **P < 0.01, ***P < 0.001

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