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Fig. 2 | BMC Cancer

Fig. 2

From: 1,5-Anhydroglucitol promotes pre-B acute lymphocytic leukemia progression by driving glycolysis and reactive oxygen species formation

Fig. 2

1,5-AG promoted pre-B ALL cell lines proliferation by enhancing glycolysis and oxidative stress. A The effects of different pre-B ALL cell lines treated with different doses of 1,5-AG for the indicated times on cell viability. B Effects of 1,5-AG on the cell cycle in pre-B ALL cell lines for 24 h. C Effects of 1,5-AG on apoptosis in pre-B ALL cell lines for 24 h. D–E Effects of 0.8 mM 1,5-AG on glycolytic rates in pre-B ALL cell lines, as determined by the Seahorse Glycolytic Rate Assay. F The lactate levels were used to verify the effect of 1,5-AG on cellular glycolysis when the pre-B ALL cell lines were treated with different doses of 1,5-AG for 24 h. G Effects of 1,5-AG on nicotinamide adenine dinucleotide phosphate levels in pre-B ALL cell lines for 24 h. H-I Reh cells and HAL-01 cells were treated with 1,5-AG, and ROS-positive cells were then measured and analyzed by flow cytometry. Data are presented as means ± standard deviations. ns (P ≥ 0.05), *P < 0.05, #P < 0.05, **P < 0.01, ##P < 0.01, ***P < 0.001

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