Fig. 4

Snail causes a partial EMT in vivo and is anti-apoptotic. Xenografts were harvested from mice 21 days post subcutaneous injection (and 14 days post-DOX induction). A Representative images of paraffin sections stained for protein shown by immunohistochemistry. n = 4 per condition. B Increased growth rate of Snail.^lo tumours. There are 30 tumours per condition at day 0 and mice were sacrificed weekly from day 14, removing 6 tumours from each cohort each time. Therefore, n = 30 at day 0 and n = 6 at day 42. Significance values based on linear regression of interaction between conditions, ***p < 0.001. The number of Bcl-2 + cells in sections of xenograft was counted (C). n = 5 biological replicates with 5 technical replicates each. Significance based on Student’s t-test, *p < 0.05