Fig. 3
Snaillo cells are motile and chemoresistant. A In a scratch wound assay, individual Snaillo cells break away from the leading edge (arrows) unlike Snailwt cell. B, C Snaillo cells show increased migration in a Transwell migration assay. Light microscopy picture of migrated cells stained with Giemsa stain 24 h after seeding in a 1%:10% serum gradient, (B). Quantification of the number of cells that had moved across the membrane (C). Error bars = SEM. -/ + DOX. n = 3. Significance based on one-way ANOVA, ***p < 0.001. (D, E) Growth curves in cells incubated with chemotherapeutic agents. Oxaliplatin (D) and 5-fluorouracil (5-FU) (E). n = 3, ****p < 0.0001, ns = not significant