Fig. 2
Changes in morphology and marker expression in DLD-1 cells following Snail induction. A Phase contrast and immunofluorescence images of cell lines with 100 ng/mL DOX for 14 days (Snailwt and Snaillo) or 4 days (Snailhi) show acquisition of mesenchymal morphology on Snail expression. B Western Blots detecting Snail and downregulation of E- cadherin. IMR90, a fibroblast line, is used as a positive control for mesenchymal proteins (Snailhi and IMR90 from a separate blot). C, D mRNA array profiling of 84 EMT regulated genes. Snail-expression leads to downregulation (C) and upregulation (D) of genes, normalised to the Snailwt. All comparisons in the presence of DOX. Error bars = 95% confidence interval. n = 3. Asterisks over data bars indicate significance from Snailwt. Asterisks over connecting lines indicate significance between Snaillo and Snail.hi. Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001