Fig. 4

SETD1A activates Notch signaling pathway. A Volcano plot of differently changed genes associated with SETD1A. B KEGG pathway of differently changed genes associated with SETD1A. C The protein levels of Nothc1, Hes1, Hey1, Hey2, and Heyl in SETD1A overexpression RMGI and OVCAR5 cells analyzed by western blotting assays. D,E The mRNA levels of Notch1, Hes1, Hey1, Hey2, and Heyl in SETD1A overexpression RMGI D and OVCAR5 E cells analyzed by RT-PCR assays. Unpaired two-tailed t-test. Data are expressed as mean ± SD. ***p < 0.001, ****p < 0.0001. F,G The protein levels of Nothc1, Hes1, Hey1, Hey2, and Heyl in SETD1A knockdown SKOV3 F and Caov3 G cells analyzed by western blotting assays. H,I The mRNA levels of Notch1, Hes1, Hey1, Hey2, and Heyl in SETD1A knockdown SKOV3 H and Caov3 I cells analyzed by RT-PCR assays. Unpaired two-tailed t-test. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001