Fig. 2
GDNF modulation using CRISPR-dCAS9 alters transcription and TAM sensitivity in TamS and TamR cells. A GDNF; p < 0.05, B EGR1; not significant, and (C) ESR1; p < 0.006, mRNA expression after endogenous upregulation of GDNF using CRISPR-dCAS9-VP64 system. D GDNF mRNA expression after endogenous downregulation of GDNF using CRISPR-dCAS9-KRAB system. E Cell viability of TamSGDNF-OE and TamSVc cells in the presence of 1 μM and 5 μM TAM, p < 0.05. F Cell viability of TamRGDNF-KD and TamRVc cells in the presence of 1 μM TAM and 5 μM (p < 0.065) TAM. Data in (F) are represented as mean ±SEM (n=2). Student’s paired t-test was used for statistical analyses