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Fig. 1 | BMC Cancer

Fig. 1

From: GDNF-RET signaling and EGR1 form a positive feedback loop that promotes tamoxifen resistance via cyclin D1

Fig. 1

CRISPR-dCAS9 provides the ability to alter gene transcription. A Schematic illustration of the derivation of Tamoxifen sensitive (TamS) and resistant (TamR) MCF-7 clonal cell lines. B As shown by the Kaplan-Meier plotter, endocrine therapy treated ERα+ BC patients with high mRNA expression of RET (red) have decreased relapse free survival (RFS) compared to patients with low RET expression (black). C Components of our dCAS9 system include the modified dCAS9 protein that no longer cuts DNA, the sgRNA used by dCAS9 to detect the target sequence, and a modulating domain to either activate (VP64) or inhibit (KRAB) gene transcription at the target site

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