Fig. 6
From: C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis
Wound healing assay, cell immunohistochemistry (IHC), and cell apoptotic assay. C/EBPβ ( +): pcDNA3.1-C/EBPβ plasmid transfection; WT: without any transfection; NC: negative control pcDNA3.1 plasmid transfection. The Student’s t-test test was used to compare the two groups of single variable data, single factor analysis of variance comparing multiple groups of data, nonparametric test to compare the variance not neat, count data using chi-square test. Ns: Not significant. *P < 0.05, **P < 0.01. All experiments were repeated three times. A, C Wound healing assay measured the effects of C/EBPβ overexpression on SiHa cell migration. Wound healing assay of SiHa cells after plasmid transfection at specified timepoints (0, 24, 48 h). B, D, E HeLa cell IHC (× 200 magnification) to measure the effects of C/EBPβ overexpression on Ki67 expression. F, G Annexin V-PI double staining for measurement of apoptosis in each treatment group. Effect of C/EBPβ overexpression on HeLa cell apoptosis. Annexin V-PI double staining of plasmid transfection of HeLa cells. Percentage of apoptotic cells in each group