Fig. 1

TGIF2 enhances LUAD cell migration and EMT in vitro. (A) GSEA of cell adhesion molecules enriched in TGIF2-low-expressing patients (upper panel). GSEA of EMT genes enriched in TGIF2-high-expressing patients (lower panel). (B) qRT-PCR analysis of EMT-related genes, including Fibronectin, u-PA, Slug, MMP9, E-cadherin, Snail, Vimentin, ZEB1, and Twist, in the indicated cells. (C) Immunofluorescence analysis of E-cadherin and Vimentin in A549 and H1299 cells with TGIF2 knockdown. Scale bars, 20 μm. (D) Western blot results of E-cadherin, Vimentin, and Fibronectin expression in TGIF2-knockdown A549 and H1299 cells. (E) Western blot results for E-cadherin, Vimentin, and Fibronectin expression in stable H1299 cells. (F) Transwell assay of the migration ability of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. (G) Wound healing assay of the migrated distance of TGIF2 knockdown A549 and H1299 cells. Scale bars, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. All data are representative of three repeated experiments