Skip to main content
Fig. 6 | BMC Cancer

Fig. 6

From: Secreted proteins MDK, WFDC2, and CXCL14 as candidate biomarkers for early diagnosis of lung adenocarcinoma

Fig. 6

Ectopic expression of MDK, WFDC2, or CXCL14 enhanced the proliferative activity of lung cancer cells. (A) Expression of MDK, WFDC2, and CXCL14 in A549 cells infected with recombinant lentivirus expressing 6xHis tagged MDK, WFDC2, or CXCL14 was determined by Western blot analysis with anti-6xHis monoclonal antibody. (B) The effect of MDK, WFDC2, or CXCL14 expression on the proliferative activity of A549 cells was assessed by CCK8 assay. Cells were infected with the corresponding lentivirus and screened by puromycin. Assays were performed at least three times. Data are expressed mean ± SEM. Kaplan-Meier survival analysis of the prognostic significance of MDK (C) WFDC2 (D) or CXCL14 (E) expression in LUAD patients using the Kaplan-Meier plotter database

Back to article page