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Fig. 1 | BMC Cancer

Fig. 1

From: Differential presence of exons (DPE): sequencing liquid biopsy by NGS. A new method for clustering colorectal Cancer patients

Fig. 1

Exploratory analysis of patients using DPE (M vs N). MA plots for selected differentially present exons combining two different methods: quasi-likelihood F-tests (QLF) (A plot) and likelihood ratio tests (LRT) (B plot) for comparative M vs N. The log-fold change (FC) ratio is plotted on the y-axis, and the average normalized counts (counts per million; CPM) is plotted on the x-axis. Differentially present exons are highlighted in red (DPE; p ≤ 0.001) and a total of 1760 exons were obtained with EdgeR. In the graph (C) we can see grouping of patients using normalized values of differentially present exons (DPE) by Ward’s method. Patients are marked with different colors according to the group in which they were included: M: red; N: green; and U: blue. Most metastatic (M) and non-metastatic (N) patients were clearly separated into two groups, while unclassifiable (U) patients were located between M and N, indicating that they share features with both groups. In the two-dimensional (D) plot, a principal component analysis (PCA) can be observed. Metastatic (M) and non-metastatic (N) patients cluster in a cloud of different points and are separated from each other, while unclassifiable (U) patients are located between M and N, probably because of their intermediate characteristics. M: red; N: green; and U: blue

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