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Fig. 3 | BMC Cancer

Fig. 3

From: Establishing a whole blood CD4+ T cell immunity measurement to predict response to anti-PD-1

Fig. 3

Gating strategy for FCM data analysis. a, f Singlet cells gated using FSC-A/FSC-H dot plot. b, g Lymphocytes gated using FSC-A/SSC-A dot plot. c, h CD4+T cells gated using CD4+/SSC-A dot plot. d, i Cells with low CD62L expression are selected as CD4+Teff cells. e, j Cells expressing both CD25 and FOXP3 are selected as CD4+Treg cells. FCM, flow cytometry; Teff, effector T cells; Treg, regulatory T cells

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