Fig. 5

The expression status of genes in the folate cycle upon MYEOV knockdown. A, The expression levels of genes associated with one-carbon metabolism, MTHFD1, MTHFD2, SHMT1, SHMT2, MTHFD1L, and MTHFD2L, were measured by qPCR in the indicated cell lines. B, The expression levels of CASTOR2 and MXD4 mRNAs were measured by qPCR. In A and B, black bars indicate cells transfected with control siRNA, and red and green bars represent cells transfected with MYEOV-targeting siRNA; TBP was used to normalize the expression levels. C, Correlations between MYEOV and MXD4 (left) or CASTOR2 (right) mRNA expression levels in the TCGA-PAAD dataset. Expression levels are shown in terms of log-transformed TPM values, and correlation coefficients and p values were calculated using Spearman’s rank correlation coefficient analysis. The dotted lines indicate the correlation regression line