Fig. 4

ACAT1 inhibits the autophagy of prostate cancer cells through FUS. A. Expression of FUS in the GEPIA database. B. Autophagy-related qPCR array. After upregulation of the expression of FUS, the changes in the expression of related genes are shown. C and D. Relationship between ACAT1 and MAP 1LC3B (P = 0.031, R = − 0.085), as well as ACAT1 and MAP 1LC3A (P = 0.31, R = 0.044), in the GEPIA database. E. In LNCaP cell line, when FUS expression increases, MAP 1LC3B mRNA expression increases accordingly. *P < 0.05, **P < 0.01. F. In PC3 cell line, when FUS expression increases, MAP 1LC3B mRNA expression increases. *P < 0.05, **P < 0.01. G. ChIP experiments demonstrate that FUS can interact with LC3B