Fig. 2

Oxaliplatin is a substrate of ABCC10 and silencing of ABCC10 sensitizes CRC cells to Oxaliplatin. a Oxaliplatin accumulation in Caco-2 cells exposed to Oxaliplatin (80 ng/mL) for 24 h is measured by UPLC-MS/MS. The remained intracellular Oxaliplatin is significantly increased when ABCC10 is knocked down by 100 nM and 200 nM of siABCC10. n = 3. ** P < 0.01, *** P < 0.001. b CRC cells treated with siABCC10 (100 nM) or siControl (100 nM) are incubated in a gradient of Oxaliplatin (10, 20, 40, 80 and 160 ng/mL) for 24 h. Oxaliplatin IC50 in siABCC10 groups is evidently decreased compared with siControl groups. n = 6. ** P < 0.01, *** P < 0.001. c Caco-2 cells are incubated in 80 ng/mL of Oxaliplatin for 24 h. Then Oxaliplatin is withdrawn and the cells are cultured for another 0, 30, 60 or 120 min. In ABCC10 knock-down cells, the intracellular Oxaliplatin residual is much more than that in controls. n = 3. *** P < 0.001