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Fig. 6 | BMC Cancer

Fig. 6

From: Exosome-transmitted circ_002136 promotes hepatocellular carcinoma progression by miR-19a-3p/RAB1A pathway

Fig. 6

MiR-19a-3p inhibition impairs the restriction of HCC progression by si-circ_002136 in vitro. A qRT-PCR was used to assess the relative expression of miR-19a-3p in specimens with different clinical stages of HCC. B Kaplan-Meier survival analysis was applied to determine the overall survival of the miR-19a-3p high and low expression groups. C Changes of intracellular miR-19a-3p in HA22T and Huh7 cells after treatment with miR-19a-3p mimic (mimic), mimic nc, miR-19a-3p inhibitor (inhibitor), or inhibitor nc (left panel). D HA22T and Huh7 cells were transfected with si-NC + inhibitor nc, si-circ_002136 + inhibitor nc, and si-circ_002136 + inhibitor, respectively, and CCK-8 was used to measure the changes of cell viability in different groups (right panel). E Flow cytometry experiment was performed to determine the changes of apoptotic activity in si-NC + inhibitor nc group, si-circ_002136 + inhibitor nc group, and si-circ_002136 + inhibitor group. F, G Transwell experiments were performed to characterize the cell migration (F) and invasion (G) abilities of si-NC + inhibitor nc group, si-circ_002136 + inhibitor nc group, and si-circ_002136 + inhibitor group. Scale bar, 50 μm. H TUNEL staining revealed the apoptotic capacity of HCC cells under different conditions. Scale bar, 50 μm. TUNEL positive cell ratio was also demonstrated (right panel). *** P < 0.001, ## P < 0.01, ### P < 0.001. *** P < 0.001 indicates compared with mimic nc group, stage I-II group and si-NC + inhibitor nc; ## P < 0.01 indicates compared with si-circ_002136 + inhibitor nc group; ### P < 0.001 indicates compared with the inhibitor nc group

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