Fig. 1

Characteristic of HCC-derived exosomes. A, B TEM and NTA were applied to identify and measure the morphology and particle size range of exosomes observed from HCC cell culture media, respectively. Scale bars, 200 nm. NTA analysis showed that the existing exosomes ranged in diameter from 100 to 200 nm. C Identification of the presence of exosomal marker proteins CD63, CD9 and Hsp70 by western blot analysis. HCC cell lysates were served as positive control. The membrane was cropped according to the molecular weight of the protein before the incubation with primary antibodies. GAPDH was used as a control. Full-length blots/gels are presented in Additional file 2. D Green fluorescent signal of internalized exosomes in HCC cells was detected by confocal laser scanning microscopy. Scale bars, 50 μm