Fig. 1From: Speckle-type POZ protein could play a potential inhibitory role in human renal cell carcinomaExpression of SPOP in ACHN, Caki-1 and Caki-2 cells. A and BÂ Relative expression of SPOP in RCC cell lines was measured by western blot analysis. SPOP expression in Caki-2 cells was highest among the examined RCC cell lines. C and DÂ SPOP was knocked down in Caki-2 cells and upregulated in ACHN and Caki-1 cells through transfection with a lentiviral vector for further experiments. The levels of SPOP in RCC cell lines were determined by western blot. ACHN and Caki-1 cells were divided into the NC group and SPOP-overexpressing group. Caki-2 cells were divided into the sh-NC and sh-SPOP groupsBack to article page