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Table 2 List of western blot primary and secondary antibodies

From: Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFβ inhibition

1° Antibody

Host

Dilution (or Concentration)

Source

Catalog numbers

anti-palladin

Rabbit

0.0002 µg/µL

Protein tech, Chicago, IL

10853-1-AP

anti-phospho SMAD2 [S465/467]/SMAD3 [S423/425]

Rabbit

0.2 µg/mL

Cell Signaling, Danvers, MA

8828s

anti-SMAD2/3 (total)

Rabbit

0.2 µg/mL

Cell Signaling, Danvers, MA

3102s

Goat anti-mouse, IRDyea

Mouse

1:10,000

LI-COR, Lincoln, NE

926-68070

Goat anti-rabbit, IRDyea

Rabbit

1:10,000

LI-COR, Lincoln, NE

926-32211

Anti-mouse IgG, HRP-linkedb

Mouse

1:5000

Cell Signaling, Danvers, MA

7076 S

Anti-Rabbit IgG, HRP-linkedb

Rabbit

1:5000

Cell Signaling, Danvers, MA

7074 S

  1. Table depicting all antibodies used denoting the antigen, host, dilution (or concentration), source, and catalog numbers
  2. aindicate secondary antibodies used in Licor
  3. bindicate secondary antibodies used in ECL