Fig. 4

Real-time polymerase chain reaction (PCR) screening of prescreening candidates in lung tumor samples. A Box plot of candidate gene expression in tumor (T39, T36, T43, T23, T35, T44) and normal (N39, N36, N43, N23, N35, N44) lung samples. CEA cell adhesion molecule 5 (CEACAM5) and high mobility group box 3 (HMGB3) have two data points for samples T43 and N43, respectively. P-value was calculated by Wilcoxon rank sum test using geom_signif function of ggplot2 package. B Expression levels of candidate genes in three tumor and three normal lung samples with individually synthesized cDNAs as template DNA. C, D Expression levels of CEACAM5 and HMGB3 in four tumor and four normal lung samples with cDNAs synthesized for C single-cell RNA sequencing (scRNA-seq) or D single-nucleus RNA sequencing (snRNA-seq). E Expression levels of candidate genes in metastatic (EBUS123) and normal (LN06) lymph node samples. F Gene expression levels of CEACAM5 and HMGB3 in human samples (four paired normal and tumor tissues) and cell line (H2228) were analyzed using the multiplex system