Table 1 Primer sequences used to generate reporter constructs
SN | Name | Sequence | Region | Cutting Site |
|---|---|---|---|---|
1 | pmiR-GLO-RAB14-both-F | TGGAAAGAGCTCCTAGCATCAG | 1989–3048 | SacI |
2 | pmiR-GLO-RAB14-both-R | TCTAGACAAGCCGCAGTCTTTAGTTTGT | 1989–3048 | XbaI |
3 | pmiR-GLO-RAB14-BS1-R | TCTAGAACAAGCGCTGAAACACCTTT | 1989–2423 | XbaI |
4 | pmiR-GLO-RAB14-BS2-F | GAGCTCTCAGCGCTTGTGCTGATACA | 2413–3072 | SacI |
5 | pmiR-GLO-RAB14-BS2-R | TCTAGAGGGAGTCAGGGTATTGCACC | 2413–3072 | XbaI |
6 | pmiR-GLO-RAB14-BS1-Del-F | GCACTGTTGCTTACCTGTTTTCTTAACTGTTCTTG | 1989–2423 | N/A |
7 | pmiR-GLO-RAB14-BS1-Del-R | CAAGAACAGTTAAGAAAACAGGTAAGCAACAGTGC | 1989–2423 | N/A |
8 | pmiR-GLO-RAB14-BS2-Del-F | CCAGGGACCATGACCTGGTGTGTGTGTATATTTAC | 2413–3072 | N/A |
9 | pmiR-GLO-RAB14-BS2-Del-R | GTAAATATACACACACACCAGGTCATGGTCCCTGG | 2413–3072 | N/A |