Fig. 6

(A) Levels of miR-214-3p in FLO-1 cells that stably overexpress miR-214-3p compared to wild- type (wt) FLO-1 cells. (B) Levels of RAB14 mRNA in the cells mentioned in (A). To generate stably expressing clones, FLO-1 cells were transduced for 48 hours with a lentiviral-miR-214-3p construct and single clones were selected using puromycin (1 μg/ml) as a selection marker. Representative bar diagrams from three separate set of experiments are shown. Each set of experiments was done in triplicate. Statistical significance is indicated by * (p < 0.05). Error bars represent mean ± S.D. (C-F) Effect of miR-214-3p in-vivo. Wt and miR-214-3p stably overexpressing FLO-1 cells were subcutaneously injected in NRG mice. Tumor volume (C), and tumor weights (D) were measured in mice for 30 days, where day 0 indicates average tumor volume for mice in wt group measured ~ 500 mm3. Levels of miR-214-3p (E) and levels of RAB14 mRNA (F) were measured in tumors generated from FLO-1 cells stably expressing miR-214-3p harvested at day 30. Statistical significance is indicated by * (p < 0.05)