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Fig. 1 | BMC Cancer

Fig. 1

From: MiR-214-3p targets Ras-related protein 14 (RAB14) to inhibit cellular migration and invasion in esophageal Cancer cells

Fig. 1

Basal levels of miR-214-3p and RAB14. Endogenous miR-214-3p expression levels measured as copy number per microliter by dd-PCR in (A) human esophageal epithelial (hESO) cells and esophageal cancer cell lines TE7, FLO-1 and SK-GT-4 and in (B) human esophageal tumor specimens with matched normal esophageal epithelial specimen, where PR = Partial response and CR = complete response. (C) Comparison of miR-214-3p expression detected by dd-PCR in the normal and tumor tissues in (B). * indicates statistical significance (p < 0.004). (D) Baseline RAB14 mRNA levels in hESO, TE7, FLO-1 and SK-GT-4 cell lines measured by q-PCR. Levels were normalized with GAPDH. Error bars represent mean ± S.D. Statistical significance is shown by *, where p < 0.05. (E) Endogenous RAB14 protein expression levels in the human esophageal cell lines. GAPDH was used as a loading control. The blot in this figure was cut prior to hybridization with antibody RAB14 and GAPDH. SI indicates relative RAB14 protein mean signal intensity measured using BioRAD Image Lab software. Signal intensity of the target protein (RAB14) is determined and is normalized by signal intensity of GAPDH. Relative signal intensity (SI) for target protein is calculated compare to hESO. (F) Representative images depicting hematoxylin and eosin (H&E) (left) and RAB14 staining (right) containing benign columnar epithelium and esophageal adenocarcinoma in human tumor samples. White arrows indicate invasive carcinoma. Black arrows indicate benign columnar epithelium. Scale bar 100 μm

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