Fig. 3
From: Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol—rRNA axis
A Methionine depletion-induced global hypomethylation. CpGi methylation per chromosome (bar plot) from reduced representation of RRBS data indicating hypermethylated (pink) and hypomethylated (green) regions in the methionine depleted compared with control state in MZGC1. q Values < 0.05 and methylation difference ≥ 25%. B Scheme of CpGi, promoters, and gene body modified from Wang et al. [2] (upper) and distribution of DMRs (lower). DMRs of CpG site are mainly intergenic regions. Total amount of CpGi and shores of DMRs was 36%. Methylation of gene body, not just promoter, accounted for a large proportion. Breakdown shows that intron > exon > untranslated regions. C Pathways for significant genes analyzed by GeneDMRs. Significant gene ontology terms and pathways (adjusted P < 0.1) of the up- and down-regulated differentially expressed genes in the hyper/hypo-methylated categories by combining with the R package clusterProfiler