Fig. 1
From: Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol—rRNA axis
A Clonogenic assay: Methionine depletion for 5 days then reseeded for 3 days markedly decreased colony formation of MZGC1 cells. Quantitative analysis of colony areas in response to methionine depletion. B Fluorescence-activated cell sorter analysis to detect cell cycle distribution. Propidium iodide (PI) staining assay using a Cell Cycle Phase Determination Kit was performed on MZGC1 cells. Cell cycle analysis showed that methionine depletion for 3 days increased sub-G1 and decreased S, G2M populations. C Dye exclusion test showed that methionine depletion for 3 days increased dead cell rate. D Fluorescence-activated cell sorter analysis using annexin-V kit showed that methionine depletion induced apoptotic cell death