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Fig. 4 | BMC Cancer

Fig. 4

From: The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells

Fig. 4

H2A.Z binds to the upstream region of the SFRP1 promoter and reduces SFRP1 expression. A Graphical representation of the CpG sites in the SFRP1 promoter. B ChIP assay of KDM1A, H3K4me1 and H3K4me2 enrichment in the P3 region upstream of the SFRP1 promoter. IgG was used as the negative control. Relative fold enrichment = [%(ChIP/Input)]/[%(IgG/Input)]. C BSP analysis of the CpG site methylation rate. Each dot represents one CpG site. White represents the absence of methylation, and pale green to deep yellow represent increasing methylation rates. D Quantitative RT–PCR analysis of the SFRP1 mRNA transcript level relative to the GAPDH mRNA transcript level in the blank control, LV-shRNA-NC, LV-shR-H2A.Z and LV-NC-H2A.Z + 5-aza-dC groups. The data are expressed as the individual mean values or the mean ± SD of each group in three independent experiments. **p < 0.01, ***p < 0.001

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