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Table 3 ChIP-PCR primer sets

From: TWIST1 activates cancer stem cell marker genes to promote epithelial-mesenchymal transition and tumorigenesis in esophageal squamous cell carcinoma

Genes

Forward Primer

Reverse Primer

Annealing

Temperature (°C)

PCR product size (bp)

TM

(°C)

SALL4 P1

ATCTCTGAGGTCTTG

GCATTGCGATTTATC

51

60

74.8

SALL4 P2

GCTTGCTTATCATTTGCCATTTC

GGATTCCTGTTCACAAAGACTG

58.5

109

72.6

CD44(S) P1

GTTATTGAGGGGAAAAAGAATGAG

GGTGGGGAGTTGGTGAATC

59

65

74.1

CD44(S) P2

GCCAAATGCCAGCCCTATG

TTCCCCTCAATAACAAGTCCAC

58

103

77.9

GDF3 P1

AAGAGAGCAATTCACAC

GCCCAACAATTCAGAG

52.3

64

74.5

GDF3 P2

GCATTCAAAGCCATCG

CATCTACATAGACCAAGC

54.7

71

77.5

NANOG P1

TCCTAAACCTCAACTTTATTCC

GCCGACTTACTACATTCTTC

56

58

75.0

MEIS1 P1

AATTAGGACTGATTCAAGGG

ATCCGCTCTGTCTTCTTC

56.2

94

75.6

MEIS1 P2

TCCTTCTCTAATCTCCTTCC

AATCAGTCCTATTCCTAATCAG

57.29

106

76.0

SOX2 P1

CCCATTTATTCCCTGAC

AGTTAATAGACAACCATCC

53

50

71.1

SOX2 P2

TTGGGTCTCCTAACTTC

TTCCGCTCTCCTCTC

54.45

83

78.5

  1. Abbreviations: P1 Primer set1, P2 Primer set2