Genes | Forward Primer | Reverse Primer | Annealing Temperature (°C) | PCR product size (bp) | TM (°C) |
---|---|---|---|---|---|
SALL4 P1 | ATCTCTGAGGTCTTG | GCATTGCGATTTATC | 51 | 60 | 74.8 |
SALL4 P2 | GCTTGCTTATCATTTGCCATTTC | GGATTCCTGTTCACAAAGACTG | 58.5 | 109 | 72.6 |
CD44(S) P1 | GTTATTGAGGGGAAAAAGAATGAG | GGTGGGGAGTTGGTGAATC | 59 | 65 | 74.1 |
CD44(S) P2 | GCCAAATGCCAGCCCTATG | TTCCCCTCAATAACAAGTCCAC | 58 | 103 | 77.9 |
GDF3 P1 | AAGAGAGCAATTCACAC | GCCCAACAATTCAGAG | 52.3 | 64 | 74.5 |
GDF3 P2 | GCATTCAAAGCCATCG | CATCTACATAGACCAAGC | 54.7 | 71 | 77.5 |
NANOG P1 | TCCTAAACCTCAACTTTATTCC | GCCGACTTACTACATTCTTC | 56 | 58 | 75.0 |
MEIS1 P1 | AATTAGGACTGATTCAAGGG | ATCCGCTCTGTCTTCTTC | 56.2 | 94 | 75.6 |
MEIS1 P2 | TCCTTCTCTAATCTCCTTCC | AATCAGTCCTATTCCTAATCAG | 57.29 | 106 | 76.0 |
SOX2 P1 | CCCATTTATTCCCTGAC | AGTTAATAGACAACCATCC | 53 | 50 | 71.1 |
SOX2 P2 | TTGGGTCTCCTAACTTC | TTCCGCTCTCCTCTC | 54.45 | 83 | 78.5 |