Table 1 Real-time PCR primer sequences
Genes | Forward primer sequence | Reverse primer sequence |
|---|---|---|
GAPDH | GGAAGGTGAAGGTCGGAGTCA | GTCATTGATGGCAACAATATCCACT |
TWIST1 | GGAGTCCGCAGTCTTACGAG | TCTGGAGGACCTGGTAGAGG |
CD44 (S) | TCCAACACCTCCCAGTATGACA | GGCAGGTCTGTGACTGATGTACA |
CD44 (V3) | GCACTTCAGGAGGTTACATC | CTGAGGTGTCTGTCTCTTTC |
CD44 (V6) | AGGAACAGTGGTTTGGCAAC | CGAATGGGAGTCTTCTCTGG |
CD44 (V8-10) | GACAGAATCCCTGCTACCAATA | ATGTGTCTTGGTCTCCTGATAA |
GDF3 | TGTCTGCCATCAAAGAAAGGGAAC | GGGACTGACCGCAACACAAAC |
BMI-1 | CGTGTATTGTTCGTTACCTGGAGAC | CATTGGCAGCATCAGCAGAAGG |
NANOG | GCAATGGTGTGACGCAGAAGGC | GCTCCAGGTTGAATTGTTCCAGGTC |
SOX2 | AACAGCCCGGACCGCGTCAA | TCGCAGCCGCTTAGCCTCGT |
KLF4 | TCTTCTCTTCGTTGACTTTG | GCCAGCGGTTATTCGG |
CRIPTO-1 | GGGATACAGCACAGTAAGGAG | ACGGTGGTAGTTCTGGAGTC |
SALL4 | CCAAAGGCAACTTAAAGGTTCAC | CCGTGAAGACCAATGAGATCTC |
MEIS 1 | ATGACACGGCATCTACTCGTTC | TGTCCAAGCCATCACCTTGCT |
ICAM | TGTGACCAGCCCAAGTTGTT | AGTCCAGTACACGGTGAGGA |
PIWIL1 | ATGATTGAAGTGGATGACAGAACTG | TACTTGACAACAGACAGACAACTAT |
SOX1 | TGAACGCCTTCATGGTGTGGTC | ATTACAAGTACCGGCCGCGC |
DPPA2 | AGAAATACAATCCAGGTCATCTACTTC | GCATATCTTGCCGTTGTTCAGG |
ABCC4 | GAAATTGGACTTCACGATTTAAGG | TTCCACAGTTCCTCATCCGT |
ABCG2 | TGAGGGTTTGGAACTGTGG | GATTCTGACGCACACCTGG |