Fig. 5

M2 macrophage induced by exosomes promote the proliferation, migration, and EMT of LSCC cells. TU212 cells and TU177 cells were co-cultured with macrophage under the treatment tumor-derived exosomes, A Migration capacity of LSCC cells(TU212 cells and TU177 cells) co-cultured with macrophage treated with exosomes was examined using the in vitro Transwell co-culture system. Representative photographs of migratory cells on the membrane coated with Matrigel (× 100 magnification) are generated. B Cell proliferation assay of LSCC cells treated with the culture medium of exosomes-treated M2 macrophage for 6 h, 12 h and 24 h. C Representative images of LSCC cell-xenografted in nude mice that resulted from co-injected with exo-treated macrophage cells or non-treated macrophage cells. D Volumes and weights of nude mice were compared after the injection of exosomes-treated or non-treated macrophages into the tumor. E The effect of the supernatants of macrophage transfected with exosomes on the EMT of LSCC cells was investigated by performing IHC. The data are expressed as mean ± SD of three independent experiments (*p < 0.01, **p < 0.05 was considered statistically significant)