Table 2 Primer sequences of the studied genes for real time qRT-PCR
Gene | Primer sequence | |
|---|---|---|
FADD | F | 5’- CTCAGGTCCTGCCAGATGAAC-3’ |
R | 5’-GGACGCTTCGGAGGTAGATG-3’ | |
c-FLIP | F | 5'-ATGTCTGCTGAAGTCAT CC-3' |
R | 5'-ATCCTCACCAATCTCCTGCC-3' | |
Caspase8 | F | 5’- AGAGTCTGTGCCCAAATCAAC-3’ |
R | 5’- GCTGCTTCTCTCTTTGCTGAA- 3’ | |
Caspase3 | F | 5'GTGGAACTGACGATGATATGGC3' |
R | 5'CGCAAAGTGACTGGATGAACC3' | |
Caspase9 | F | 5′-AACCCTAGAAAACCTTACCCC-3′ |
R | 5′-CATCACCAAATCCTCCAGAAC-3′ | |
Bax | F | 5'-ATGGCTTCTATGAGGCTGAG-3' |
R | 5'-CGGCCCCAGTTGAAGTTG-3' | |
Bcl-2 | F | 5'-CTGCACCTGACGCCCTTCACC-3' |
R | 5'-CACATGACCCCACCGAACTCAAAGA-3' | |
TNF-A | F | 5'-CTGAACTTCGGGGTGATCG-3' |
R | 5'-GCTTGGTGGTTTGCTACGAC-3' | |
GAPDH | F | 5'-GTCTCCTCTGACTTCAACAGCG-3' |
R | 5'-ACCACCCTGTTGCTGTAGCCAA-3' | |