Fig. 5

RAC1 is a direct target of miR-142-3p and miR-142-5p. A The complementary sequences of miR-142-3p and RAC1 or miR-142-5p and RAC1 were predicted by the STARBASE database. B The relationship between miR-142-3p and RAC1 or miR-142-5p and RAC1 was calculated by luciferase reporter assay. ^**P < 0.01, miR-NC vs. miR-142-3p/miR-142-5p group. C The mRNA expression levels of RAC1 in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + miR-142-3p inhibitor; sh-circBRWD3 + miR-142-5p inhibitor) were determined by qRT‐PCR. ^**P < 0.01, Sh-NC vs. Sh-circ group; ^{^^}P < 0.01, Sh-circ vs. Sh-circ + 3p inhibitor/Sh-circ + 5p inhibitor. D The protein levels of RAC1 in breast cancer cell lines (MDA-MB-231 as well as BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + miR-142-3p inhibitor; sh-circBRWD3 + miR-142-5p inhibitor) were calculated by western blot. E The mRNA expression levels of RAC1 in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + OE-vector; sh-circBRWD3 + OE-RAC1) were assessed by qRT‐PCR. ^**P < 0.01, Sh-NC vs. Sh-circ group; Sh-circ + vector vs. Sh-circ + oe-RAC1. F The protein levels of RAC1 in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + OE-vector; sh-circBRWD3 + OE-RAC1) were determined using western blot. G The colony numbers of breast cancer cells (MDA-MB-231 as well as BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + OE-vector; sh-circBRWD3 + OE-RAC1) were calculated by colony formation assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; Sh-circ + vector vs. Sh-circ + oe-RAC1. H The cell migration in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + OE-vector; sh-circBRWD3 + OE-RAC1) was examined by transwell assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; Sh-circ + vector vs. Sh-circ + oe-RAC1. All experiments were carried out in triplicate. Sh-circ: sh-circBRWD3; Oe-EIF4A3: EIF4A3 overexpression