Fig. 4

CircBRWD3 functions as a sponge of miR-142-3p and miR-142-5p. A Putative targets of wild-type and mutant circBRWD3 on miR-142-3p and miR-142-5p. B The relationship between circBRWD3 and miR-142-3p or circBRWD3 and miR-142-5p was calculated by luciferase reporter assay. ^**P < 0.01, miR-NC vs. miR-142-3p/miR-142-5p group. C The relationship between circBRWD3 and miR-142-3p or circBRWD3 and miR-142-5p was calculated by RNA pull-down assay. ^**P < 0.01, NC-probe vs. circ-probe group. D The expression levels of miR-142-3p and miR-142-5p in breast cancer cell lines (MDA-MB-231 as well as BT-549) transfected with OE-vector or OE-circBRWD3 plasmids and sh-NC or sh-circBRWD3 were calculated by qRT‐PCR. ^**P < 0.01, Sh-NC vs. Sh-circ group; mock vs. circBRWD3 group. E The cell proliferation in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + miR-142-3p inhibitor; sh-circBRWD3 + miR-142-5p inhibitor) were calculated using CCK-8 assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; ^{^^}P < 0.01, Sh-circ vs. Sh-circ + 3p inhibitor/Sh-circ + 5p inhibitor. F The colony number of breast cancer cells (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + miR-142-3p inhibitor; sh-circBRWD3 + miR-142-5p inhibitor) were calculated by colony formation assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; ^{^^}P < 0.01, Sh-circ vs. Sh-circ + 3p inhibitor/Sh-circ + 5p inhibitor. G The cell migration in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with indicated constructs (sh-NC; sh-circBRWD3; sh-circBRWD3 + miR-142-3p inhibitor; sh-circBRWD3 + miR-142-5p inhibitor) were calculated by transwell assay. ^**P < 0.01, Sh-NC vs. Sh-circ group; ^{^^}P < 0.01, Sh-circ vs. Sh-circ + 3p inhibitor/Sh-circ + 5p inhibitor. All experiments were carried out in triplicate. Sh-circ: sh-circBRWD3; circ-probe: circBRWD3-probe