Fig. 3

EIF4A3 upregulates circBRWD3 in breast cancer cells. A Schematic representation of the target region of EIF4A3 in the upstream region of circBRWD3 pre mRNA. B The relationship between EIF4A3 and circBRWD3 was confirmed by the RNA immunoprecipitation (RIP) assay. ^**P < 0.01, Anti-IgG vs. Anti-EIF4A3 group. C The expression level of EIF4A3 in breast cancer cell lines (MDA-MB-231 as well as BT-549) transfected with OE-vector or OE-EIF4A3 plasmids and sh-NC or sh-EIF4A3 was determined using qRT‐PCR. ^**P < 0.01, Sh-NC vs. Sh-EIF4A3 group; vector vs. Oe-EIF4A3 group. D The mRNA expression level of circBRWD3 in breast cancer cell lines (MDA-MB-231 as well as BT-549) transfected with OE-vector or OE-EIF4A3 plasmids and sh-NC or sh-EIF4A3 was determined using qRT‐PCR assay. ^**P < 0.01, Sh-NC vs. Sh-EIF4A3 group; vector vs. Oe-EIF4A3 group. All experiments were carried out in triplicate. Oe-EIF4A3: EIF4A3 overexpression