Fig. 4

Development of lymph node metastasis model. A Tumor size corresponding to invasive and wild-type KYSE-510 cells (p = 0.025). B Tumor mass corresponding to invasive and wild-type KYSE-510 cells (p = 0.070). C Subcutaneous tumor established via xenograft injection. D Xenograft extracted and excised into 2 × 2 mm pieces, visualized with GFP. E Subdivided xenograft transplanted and grown in mouse elbow, visualized with GFP. F Axillary lymph node metastasis via lymphatic vessels. (* p < 0.05 versus wild-type)