Fig. 3

NSMCE2 and MAL2 RNA expression is regulated by SEs in breast cancer cells. Blocking BRD4 binding to SEs with BET inhibitors reduces gene expression for NSMCE2 and MAL2 starting at 6 h in most breast cancer cell lines tested. MCF7, HCC1954, MDA-MB-231, Hs578T and BT-549 breast cancer cells were treated with vehicle (DMSO), 1 μM JQ1 or 1 μM IBET-151 for 6 and 24 h. After treatment, changes in gene expression levels were analyzed by qPCR. ANOVA followed by Dunnett’s multiple comparison test was performed, *P < 0.05, **P < 0.01, ***P < 0.001, ****P <  0.0001