Fig. 2From: Adjuvant effect of IRES-based single-stranded RNA on melanoma immunotherapyCrPVIRES-ssRNA activates dendritic cells in drain inguinal lymph nodes. A Mouse bone marrow-derived dendritic cells (BMDCs) were stimulated with CrPVIRES-ssRNA or poly(I:C) for 24 h. Cell activation was measured by flow cytometry using antibodies specific to CD40, CD80, and CD86. B Mice were intramuscularly immunized once with poly(I:C) (20 µg) and CrPVIRES-ssRNA (20 µg and 100 µg) for 24 h (n = 5). C Percentages of co-stimulatory molecules (CD40, CD80, and CD86) of CD11c+ DCs in lymphocytes isolated from drain inguinal lymph nodes. D, E Percentages of activation markers of DCs (cDC1, cDC2, pDC) in CD40 + cells (D) and CD80 + cells (E). Dendritic subsets were analyzed by flow cytometry using CD8a+, CD8−, and B220.+. Data were represented as mean ± SD. Statistical significance was indicated by *p < 0.05, **p < 0.01, and ***p < 0.005Back to article page