Fig. 4

RPL34-AS1 regulated ACAA2 expression through miR-575. A The targeted genes expression was detected by RT-qPCR after knockdown or overexpression of lncRPL34-AS1 in EC109 cells. B The expression of ACAA2 was performed by RT-qPCR in ESCC tissues. C Prediction for miR-575 binding elements on ACAA2. D Mutations were generated in ACAA2 3′-UTR binding sites of miR-575. DLR analysis was performed to confirm miR-575 shares binding sites with ACAA2. The luciferase activity was detected in cells co-transfected with miR-575 mimics/miR-NC and wild-type/mutant ACAA2; and E to further evaluate the relationship of RPL34-AS1, miR-575, and ACAA2 by use of DLR analysis. Data were showed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001