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Fig. 3 | BMC Cancer

Fig. 3

From: Clinical significance and oncogenic function of NR1H4 in clear cell renal cell carcinoma

Fig. 3

NR1H4 regulates the expression of CCNE2, CDK2. A Cell cycle was enriched in the NR1H4 high expression group of ccRCC. B The correlation analysis for NR1H4 and the Cyclins family members in TCGA database. C The mRNA levels of CCNE2 was downregulated in NR1H4 knockdown cells by qRT-PCR assays. The protein levels of CCNE2 was downregulated in NR1H4 knockdown cells by western blot (D) and immunofluorescence (E). F The distribution of cells in different cell cycle stages was analyzed by Flow Cytometry. G Scatterplots of correlations between NR1H4 expression and CDK2 which derived from an analysis of the TCGA dataset. (H, I) qRT-PCR and western blot revealed that knockdown of NR1H4 inhibited CDK2 mRNA and protein level in 786-O cells. CCNA2, cyclin A2; CCNB1, cyclin B1; CCNB2, cyclin B2; CCNB3, cyclin B3; CCND1, cyclin D1; CCND2, cyclin D2; CCND3, cyclin D3; CCNE1, cyclin E1; CCNE2, cyclin E2; CDK2, cyclin dependent kinase 2; DAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; *p < 0.05; **p < 0.01; ***p < 0.001

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