Fig. 3

Knockdown of CTGF or BAMBI reduces the expression of cell cycle regulators. A, C, E, G, Bar graphs showing relative mRNA levels of cyclin D1, cyclin D3, CDK2, and CDK4 were estimated by RT-qPCR in Y-MESO-14 and Y-MESO-27 cells transfected with BAMBI siRNA (A-D) or CTGF siRNA (E–H) compared with cells transfect control siRNA. Results are expressed as mean ± SD of three independent experiments (*p < 0.05 by two-tailed Student’s t-test). B, D, F, H, Cyclin D1, cyclin D3, CDK2, and CDK4 protein expression by mesothelioma cells following BAMBI or CTGF knockdown. Both cyclin D3 and CDK2 expression levels were reduced by either BAMBI or CTGF knockdown