Fig. 1From: BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferationKnockdown of CTGF reduces MM cell proliferation rate and suppress the expression of BAMBI. A, Western blots of MM cell lines NCI-H28, MSTO-211H, Y-MESO-8D, Y-MESO-14, Y-MESO-27, NCI-H2052, and NCI-H2452. Antibodies against actin and CTGF were used as the primary antibodies. B, Bar graphs of relative expression levels of CTGF in Y-MESO-14 and Y-MESO-27 cells transfected with a CTGF-targeted siRNA (20 nM) and compared with CTGF levels in non-transfected cells and cells transfect with control siRNA. The blots showing protein levels assessed by western blotting in control cells and cells transfected CTGF-targeted siRNA. C, Graphs of Proliferation rates as measured by viable cell counting assay. D, Graphs of relative mRNA levels and a western blot of BAMBI levels in control cells and cells transfected with CTGF-targeted siRNA. All results are expressed as mean ± SD of three independent experiments (*p < 0.05 vs. control siRNA group by one-way ANOVA with post hoc Bonferroni tests for pair-wise comparisons). Original unprocessed western blots are included in (Supplementary file, original blots)Back to article page