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Fig. 1 | BMC Cancer

Fig. 1

From: BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Fig. 1

Knockdown of CTGF reduces MM cell proliferation rate and suppress the expression of BAMBI. A, Western blots of MM cell lines NCI-H28, MSTO-211H, Y-MESO-8D, Y-MESO-14, Y-MESO-27, NCI-H2052, and NCI-H2452. Antibodies against actin and CTGF were used as the primary antibodies. B, Bar graphs of relative expression levels of CTGF in Y-MESO-14 and Y-MESO-27 cells transfected with a CTGF-targeted siRNA (20 nM) and compared with CTGF levels in non-transfected cells and cells transfect with control siRNA. The blots showing protein levels assessed by western blotting in control cells and cells transfected CTGF-targeted siRNA. C, Graphs of Proliferation rates as measured by viable cell counting assay. D, Graphs of relative mRNA levels and a western blot of BAMBI levels in control cells and cells transfected with CTGF-targeted siRNA. All results are expressed as mean ± SD of three independent experiments (*p < 0.05 vs. control siRNA group by one-way ANOVA with post hoc Bonferroni tests for pair-wise comparisons). Original unprocessed western blots are included in (Supplementary file, original blots)

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