Fig. 3
EVs from TNBC cells induced platelet aggregation. Representative traces from light transmission aggregometry showing platelet aggregation induced by EVs at various concentrations (A) Statistical analysis of the effect of EVs from TNBC cells on platelet function. Data generated with Hs578T EVs and Hs578Ts(i)8 EVs was co-analysed together to determine if there were significant differences between the effect of EVs isolated from the conditioned media from the cell line variants tested. Graphs represent mean ± SEM from n = 4 independent experiments. One-way ANOVA was carried out for (B) and (C) and a two-way ANOVA for (D). ****p < 0.0001 vs control (platelets stirred in the absence of EVs). Representative micrographs from optical microscopy demonstrating the presence of platelet aggregates in the presence of EVs at different concentrations. Scale bar 200 μm (E). For full two-way ANOVA results see Supplemental Material