Fig. 4

(A-B). The IGF-1-SRPK2 AXIS PROMOTE FASN mRNA stability. A MDA-MB-231 and MCF-7 cells were serum starved overnight with vehicle or SRPK2 inhibitor (3 μM) followed by exposure to IGF-1 (100 ng/mL) and with Actinomycin-D (5 μg/ mL) for 0, 3, 5 hours in full growth media, prior to RT-qPCR analysis. All values were standardized to actin. Graphs represent a relative fold change to baseline (0 hours of actinomycin-D) are expressed as means of three independent trials (n = 3) ± SD. Students T test were performed to determine significance. “*” = p ≤ .05, “**” = p ≤ .01, “N.S” = p ≥ .05. B MDA-MB-231 cells were transfected with control or SRPK2 siRNA for 48 hours and serum starved for 24 hours with or without IGF-1 (100 ng/mL) exposure. RNA was subjected to RT-qPCR with FASN intron or exon specific primers. Significance testing were done using the mean folds of three independent trials (n = 3) ± S.E.M. Student’s T test was performed for statistical analysis