Fig. 5

Over expressed MAPKs proteins followed by analyzing up regulated proteins in GO and KEGG pathway in HT29-Nrf₂⁻ cells. A Proteins involved in activating MAPK signaling pathway and noncanonical Notch pathway. B ribosomal proteins and (C) transcriptional regulators as Log₂ fold change (Log₂FC) in Nrf₂ knocked down cells. D Validation of MAPKs related proteins and downstream proteins (ERK1/2, Raf1 and FOXO3a) using Western blot analysis. As shown, MAPKs proteins were overexpressed while the expression of MAPKs negative regulators decreased. Results were expressed as the mean ± SD. (*p < 0.05, **p < 0.01, and ***p < 0.001)