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Fig. 5 | BMC Cancer

Fig. 5

From: Paradoxical downregulation of LPAR3 exerts tumor-promoting activity through autophagy induction in Ras-transformed cells

Fig. 5

The effect of LPAR3 KO on the survival and migration of Bhas 42 cells. A Cell viability was determined in parental and LPAR3 KO Bhas 42 cells without any treatment for the indicated days. Data was expressed as the mean ± SD of quadruplicate determinations. B Parental and LPAR3 KO Bhas 42 cells were cultured in the presence of lithocholic acid as tumor promotor (days 4–14). After 21 d, the cells were fixed, and Giemsa-stained to visualize malignant foci. Transformation frequency was expressed as the mean ± SD of sextuplicate determinations. C For anchorage-independent colony-formation assay, cells were grown in soft agar for 3 weeks. A representative photograph showing anchorage-dependent colony formation by parental and LPAR3 KO Bhas 42 cells. The right panel shows the average numbers of colonies per plate. Assays were performed in triplicate. All the colonies or colonies > 0.3-mm in size in each well were counted under a microscope (× 10 magnification). **P < 0.01 compared to parental NIH 3 T3 cells

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