Fig. 3From: Paradoxical downregulation of LPAR3 exerts tumor-promoting activity through autophagy induction in Ras-transformed cellsGeneration of the LPAR3 knockout in NIH 3 T3 cells. A LPAR3 sg RNA and target site were shown. B, C Generation of LPAR3 KO was confirmed by RT-PCR (B) and sequencing target site (C). D Representative immunofluorescence images of LPAR3 KO and its parental NIH 3 T3 cells stained with Hoechst 33342 (nuclear stain [blue]) or antibodies selective against LPAR3 (red). DSB, double strand breaks; PAM, protospacer adjacent motifBack to article page