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Fig. 3 | BMC Cancer

Fig. 3

From: An experimental model for ovarian cancer: propagation of ovarian cancer initiating cells and generation of ovarian cancer organoids

Fig. 3

iOVCAR-3-OSKM showed SP enrichment and malignant properties. A The heatmap of DEGs. The row displays genes, and the column represents samples. Downregulated genes are displayed in green, and genes upregulated are displayed in red. The brightness of each color corresponds to the magnitude of the difference compared with the average value. B Validation of NGS data with q-PCR data by log2 gene expression fold changes. Error bars indicate SEM. (C) GO enrichment analyses of the intersecting DEGs of the iOVCAR-3-OSKM/OVCAR-3 and SP/OVCAR-3 datasets. Count represents the number of DEGs enriched in each term. The black trend line represents the log10 (P value). D Efflux activity of cells. The SP cells unlabeled by Hoechst 33,342 without VM and with 50 µM and 250 µM VM were counted. The left panel shows representative dot plots of labeled and unlabeled cells. The right panel shows statistics for SPs in each group (n = 3). Error bars indicate SD. *P < 0.05, Student’s t test. E Sensitivity to chemotherapeutic agents. The viability of the cells without PTX treatment was assumed to be 100%, and the viability of cells with PTX treatment was normalized to this value (n = 3). Error bars indicate SD. *P < 0.05, **P < 0.005, Student’s t test. F The expression of EMT-related genes. The endogenous EMT-related genes and the housekeeping gene GAPDH were amplified and separated by electrophoresis. Neg Ctl: negative control (PCR mixture without cDNA). The samples derive from the same experiment and gels were processed in parallel. G Migration ability of iOVCAR-3-OSKM cells. A total of 1 × 105 cells were seeded into the upper layer of a cell culture insert with serum-free medium, and medium containing 10% FBS was placed below the permeable membrane for 24 h of incubation. The left panel shows images of migrating cells. The right panel provides statistics for migrating cell numbers (n = 3). Error bars indicate the SD. *P < 0.05, Student’s t test

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