Fig. 5

Construction of NudCD1 overexpression plasmid and validation. cDNA NudCD1 primer PCR electrophoresis, the size of PCR products were consistent with the open reading frame (ORF) of NudCD1(1752 bp) (A). NudCD1 colony PCR products, four lanes were positive (B). PCR positive colonies were verified by sequencing extracted plasmid. 293 T cells infected with lentivirus plasmid for 48 h (C), lentivirus was observed by fluorescence microscopy 48 h after transfection, indicating that lentiviral production was in good condition. NudCD1 overexpressed lentivirus infected colon cancer cells HT-29 (bright-field and fluorescence) (D). Empty viral vector infected colon cancer cells HT-29 (bright-field and fluorescence) (E)