Fig. 6
From: Lnc-SELPLG-2:1 enhanced osteosarcoma oncogenesis via hsa-miR-10a-5p and the BTRC cascade
Validation of the target miRNAs and mRNAs of lnc-SELPLG-2:1. A RT-qPCR in validation of the target miRNAs of lnc-SELPLG-2:1 in SaOS2 cells. B Validation of target mRNA in SaOS2 cells. C Validation of the target microRNAs, hsa-hsa-miR-10a-5p and hsa-hsa-miR-184, and messenger RNAs, AKT2 and BTRC, in tumor tissues formed in vivo. D Dual-luciferase assay for validating lncRNA-miRNA and miRNA-mRNA interactions. E hsa-miR-10a-5p targets lnc-SELPLG-2:1 and BTRC for their binding sites and corresponding mutant region. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)